Journal: PLoS ONE

Article Title: Regeneration of esophagus using a scaffold-free biomimetic structure created with bio-three-dimensional printing

doi: 10.1371/journal.pone.0211339

Figure Lengend Snippet: The bio-3D printed structures are shown before transplantation. A-D: HE staining. E-H: Immunohistochemical staining with anti-αSMA antibodies. I-L: Immunohistochemical staining with anti-VEGF antibodies. M-P: immuno-histochemical staining with anti-CD31 antibodies. Q-T: Immunohistochemical staining with anti-elastin antibodies. Scale bar = 100 μm. HE: hematoxylin-eosin, SMA: smooth muscle actin, VEGF: vascular endothelial growth factor.

Article Snippet: Immunohistochemistry was performed with the following primary antibodies: alpha smooth muscle antibody (αSMA; 1:200; mouse monoclonal; A5228; SIGMA-ALDRICH, St. Louis, MO, USA) for evaluation of the smooth muscle cell distribution, anti-CD31 (1:400; rabbit polyclonal; bs-195R; Bioss, Boston, MA, USA) for evaluation of the endothelial cell distribution, anti-vascular endothelial growth factor (VEGF; 1:200; rabbit polyclonal; sc-152; Santa Cruz, Texas, USA) for evaluation of vasculogenesis, elastin (1:200; rabbit polyclonal; ab21610; Abcam, Cambridge, UK) for evaluation of generation of ECM, anti-pan-cytokeratin (1:200, mouse monoclonal; ab7753; Abcam) for evaluation of epithelialization, and anti-HLA class I ABC antibody (1:20, mouse monoclonal; ab70328; Abcam) for evaluation of distribution of implanted structure made of human cells.

Techniques: Transplantation Assay, Staining, Immunohistochemical staining

Journal: Laboratory investigation; a journal of technical methods and pathology

Article Title: Merkel Cell Carcinoma Expresses Vasculogenic Mimicry: Demonstration in Patients and Experimental Manipulation in Xenografts

doi: 10.1038/labinvest.2014.99

Figure Lengend Snippet: Biomarkers employed for identification of VM versus true angiogenesis.

Article Snippet: All sections were incubated overnight with primary antibodies at room temperature (~25°C) followed by 2-hour incubation with HRP-conjugated goat anti-rabbit (for Ulex, human and mouse CD31, CD144 and laminin), horse anti-goat (for VEGFR-1) and horse anti-mouse (for Nodal) secondary antibodies (all Vector Laboratories, Burlingame, CA) at room temperature.

Techniques:

Journal: Laboratory investigation; a journal of technical methods and pathology

Article Title: Merkel Cell Carcinoma Expresses Vasculogenic Mimicry: Demonstration in Patients and Experimental Manipulation in Xenografts

doi: 10.1038/labinvest.2014.99

Figure Lengend Snippet: WaGa and MKL-1-derived xenograft tumors contained CD31 − anastomosing networks that were enhanced with PAS staining, and were associated with staining for laminin (Lam), CD144, Nodal and VEGFR-1. Note the density and complexity of laminin + structures in comparison to CD31 + vessels (CD31 and Lam panels original magnification, 200×; PAS, CD144, Nodal, and VEGFR-1 original magnification, 1000×).

Article Snippet: All sections were incubated overnight with primary antibodies at room temperature (~25°C) followed by 2-hour incubation with HRP-conjugated goat anti-rabbit (for Ulex, human and mouse CD31, CD144 and laminin), horse anti-goat (for VEGFR-1) and horse anti-mouse (for Nodal) secondary antibodies (all Vector Laboratories, Burlingame, CA) at room temperature.

Techniques: Derivative Assay, Staining

Journal: Laboratory investigation; a journal of technical methods and pathology

Article Title: Merkel Cell Carcinoma Expresses Vasculogenic Mimicry: Demonstration in Patients and Experimental Manipulation in Xenografts

doi: 10.1038/labinvest.2014.99

Figure Lengend Snippet: MKL-1 tumors treated with vehicle, carboplatin and etoposide stained for CD31 (upper panels; original magnification, 100×) and laminin (Lam) (middle panels; original magnification, 100×); rectangular regions are representative fields enlarged for clarity (original magnification, 1000×). Note the marked and widespread increase in laminin + networks after chemotherapy. A similar picture is observed in WaGa derived tumors, although less diffusely (data not shown). A statistically significant increase in laminin immunoreactivity after carboplatin is observed in both cell lines, and after etoposide in MKL-1 derived tumors (graphs, lower panels).

Article Snippet: All sections were incubated overnight with primary antibodies at room temperature (~25°C) followed by 2-hour incubation with HRP-conjugated goat anti-rabbit (for Ulex, human and mouse CD31, CD144 and laminin), horse anti-goat (for VEGFR-1) and horse anti-mouse (for Nodal) secondary antibodies (all Vector Laboratories, Burlingame, CA) at room temperature.

Techniques: Staining, Derivative Assay

Journal: Laboratory investigation; a journal of technical methods and pathology

Article Title: Merkel Cell Carcinoma Expresses Vasculogenic Mimicry: Demonstration in Patients and Experimental Manipulation in Xenografts

doi: 10.1038/labinvest.2014.99

Figure Lengend Snippet: Relative LAMA3 (left), LAMB3 (center, left), LAMC2 (center, right) and CD144 (right) mRNA expression (mean ± s.e.m.) by carboplatin- and etoposide-resistant versus vehicle-treated MKL-1 (top) and WaGa (bottom) cells, as determined by real-time quantitative RT-PCR. Established human umbilical vein endothelial cells (HUVEC) served as a positive control. Data are representative of n=3 independent experiments. P<0.05, ** P<0.01, *** P<0.001).

Article Snippet: All sections were incubated overnight with primary antibodies at room temperature (~25°C) followed by 2-hour incubation with HRP-conjugated goat anti-rabbit (for Ulex, human and mouse CD31, CD144 and laminin), horse anti-goat (for VEGFR-1) and horse anti-mouse (for Nodal) secondary antibodies (all Vector Laboratories, Burlingame, CA) at room temperature.

Techniques: Expressing, Quantitative RT-PCR, Positive Control

Journal: Laboratory investigation; a journal of technical methods and pathology

Article Title: Merkel Cell Carcinoma Expresses Vasculogenic Mimicry: Demonstration in Patients and Experimental Manipulation in Xenografts

doi: 10.1038/labinvest.2014.99

Figure Lengend Snippet: PAS-positive networks also are demonstrable by laminin IHC, and a minority of these are associated with CD31 staining in adjacent sections original magnification, 100×; inset, 1000×). Double IF labeling for CD31 and CD144 demonstrates an architectural pattern of CD144 positivity similar to that seen with laminin, and distinct from the comparatively few and discrete CD31 + vessels (original magnification, 200×)

Article Snippet: All sections were incubated overnight with primary antibodies at room temperature (~25°C) followed by 2-hour incubation with HRP-conjugated goat anti-rabbit (for Ulex, human and mouse CD31, CD144 and laminin), horse anti-goat (for VEGFR-1) and horse anti-mouse (for Nodal) secondary antibodies (all Vector Laboratories, Burlingame, CA) at room temperature.

Techniques: Staining, Labeling

Journal: Frontiers in Cell and Developmental Biology

Article Title: Prostate-Specific Membrane Antigen (PSMA) Promotes Angiogenesis of Glioblastoma Through Interacting With ITGB4 and Regulating NF-κB Signaling Pathway

doi: 10.3389/fcell.2021.598377

Figure Lengend Snippet: PSMA is abundantly expressed in neurovascular structure of GBM and predicts poor prognosis. (A) IHC was performed to detect PSMA expression in normal and GBM tissues and representative images of GBM tissues with faint, moderate and maximum PSMA expression were shown (Scale bar = 100 μm). (B) The Immunofluorescence colocalization detection of CD31 and PSMA in GBM tissues (Scale bar = 100 μm). (C) Kaplan-Meier survival analysis was performed to reveal the significance of PSMA expression in prognosis of GBM patients.

Article Snippet: Next, all slides were blocked by 3% H 2 O 2 for 25 min in dark, then incubated with 3% BSA or rabbit serum for 30 min. Primary antibodies CD31 (# GB13063, Proteintech 1: 200), PSMA (# ab19071, Abcam, 1:100) were applied at 4°C overnight.

Techniques: Expressing, Immunofluorescence